DFCCIL Question-Paper Contributed by Dinesh Kumar updated on Apr 2019

 


Dedicated Freight Corridor Corporation of India (DFCCIL)

1. Dissolving salt in water is an example of which type of change ? 

Physical-Answer

Nuclear

Chemical

Biochemical

2. When nuclear change takes place, which is true 

Energy is destroyed

Energy is released-Answer

Energy is changed

None of these

3. Which type of radiation is unaffected by magnetism ? 

Alpha

Beta

Gamma-Answer

All of these

4. Which temperature scale is an absolute scale, where the zero point is the lowest possible temperature ? 

Kelvin-Answer

Celsius

Fahrenheit

Centigrade

5. The relative randomness of molecules in the states of matter is best indicated by 

Solid > Liquids > Gas-Answer

Liquid > Gas > Solid

Liquid > Solid > Gas

Gas > Liquid > Solid

6. Whether or not two liquids are miscible is determined by 

Molecular Size : Molecules of similar sizes tend to mix together

Density : Liquids of simliar densities usually mix well together

Polarity : Polar and nonpolar liquids are not miscible while polar liquids can usually be mixed with each other-Answer

All of the above factors directly affect miscibility

7. The Triple Point is 

The combination of standard temperature, pressure and volume

The combination of temperature and pressure at which the solid, liquid and gas phases can coexist at equilibrium

The combination of pressure and temperature at which liquid, gas, and plasma phases all exist in equilibrium-Answer

The combination of temperature and pressure at which liquids and gases cannot be distinguished from each other


8. If the work done by a force depends only on the initial and final stages and not on the path taken, then this type of force will be 

Conservative Force-Answer

Non-conservative Force

Conservative as well as Non-conservative force

None of these

9. Working between which pair of following temperatures, the efficiency of a carnot engine will be maximum ? 

10 Degrees F and 10 Degress R-Answer

10 Degrees F and 10 Degress C

10 Degrees R and 10 Degress C

It will be same in all the above cases

10. Dacron is a 

Polyester-Answer

Polyamide

Nylon

Resin

11. Which of the following has the highest stable atom ? 

Bi

Al

Cl

Pb-Answer

12. In Friedel-Crafts reaction, AlCl3 acts as a 

Lewis Acid-Answer

Lewis Base

Catalyst

Reactant

13. The number of unpaired electrons in [Ni(CO)4] is 

Zero-Answer

One

Three

Five

14. Iodoethane reacts with sodium to form 

Pentane

Propane

Butene

Butane-Answer

15. An ideal gas has maximum density at 

1 atm, 300 K

2 atm, 150 K-Answer

0.5 atm, 600 K

1 atm, 500 K


16. Which of the following metals is present in brass, bronze and german silver ? 

Defence Mechanism

Hydrolysis of ATP

Trifling of Contraction-Answer

Generating right electrical potential across cell membrane

17. First ionization potential is ___________ second ionization potential. 

Less than-Answer

More than

Equal to

None of these

18. n-propyl benzene is best prepared by 

Friedel-Crafts Reaction

Wurtz Reaction

Wurtz-Fitting Reaction-Answer

Grignard Reaction

19. Chloroform is slowly oxidized by air to 

Formly Chloride

Carbonyl Chloride-Answer

Trichloroacetic Acid

Formic Acide

20. Electrophoresis is 

Movement of colloidal particle under the influence of electric field-Answer

Movement of colloidal particle under the influence of magnetic field

Movement of colloidal particle in zig-zag fashion

Scattering of light by colloidal particles

21. Rosenmund's reduction of an acyl chloride gives 

Alcohol

Ester

Hydrocarbon

Aldehyde-Answer

22. Pyrophosphoric acid is 

Monobasic

Dibasic-Answer

Tribasic

Basic

23. Which of the following is an example of a mathematical model ? 

Area of a circle = PI x Radius^2

Volume of a cube = Length^3

Force = Mass x Acceleration

All of the above-Answer

24. The specific activity of an enzyme is relatively constant between pH 3-8. If the enzyme activity at pH 7.0 is to be modeled, which of the following would be a reasonable simplifying assumption ? 

Specific activity is not dependent on temperature

Specific activity is not dependent on substrate concentration

Specific activity is not dependent on pH-Answer

All of the above

25. The bioreactor which is most commonly used in industry is the 

Batch Fermenter

Fed Batch Fermenter-Answer

Continous Fermenter

Batch Continous Fermenter

26. Which of the following is correct with regards to microbial growth ? 

Cells are autocatalysts and thus the rate of increase in biomass (dX/dt) keeps changing

Many factors affect cell growth

The rate of increase in biomass concentration is not constant during a fermentation

All of the above are correct-Answer

27. During a batch fermentation, when would the specific growth rate be lowest ? 

During exponential phase

During the lag and stationary phase-Answer

When cells are growing at their fastest rate

Throughout the fermentation

28. Which of the following describes a limitation of the exponential growth model ? 

Biomass growth is assumed to be not linked to the availability of nutrients or substrates

It does not predict when cell growth will cease

It predicts that all types of cells under all conditions will grow exponentially

All of the above are correct-Answer

29. In large scale bioreactors, dissolved oxygen levels are often so low that they restrict the rate of cell growth. In this case 

Dissolved oxygen would be the growth limiting nutrient

The nitrogen source would be the growth limiting nutrient

The carbon source would be the growth limiting nutrient-Answer

Dissolved oxygen levels would not be the growth limiting nutrient

30. Which of the following is not correct ? 

The Monod model is an empirical correlation

The Michaelis Menten model is a mechanistic model

The Monod model was derived from a study of the mechanism of microbial growth-Answer

The Michaelis Menten model was derived from a study of the mechanism of enzyme action

31. The technique of Nick Translation was developed by 

Rigby and Paul Berg-Answer

Winkler

Roderick and Rigby

None of them

32. Which technique allows knowing the status in the interphase ? 

Hybridization Microarray

Karyotyping

Fluorescence in situ Hybridization-Answer

Pedigree Analysis

33. _________ observed that protein sequences undergo variation during evolution according to certain patterns 

Zuckerkandl

Pauling

Margaret Dayoff-Answer

All of them

34. Which of the following would have the highest oxygen transfer rate characteristics ? 

A sparged stirred tank bioreactor being stirred at 200 rpm

A non-sparged stirred tank bioreactor being stirred at 200 rpm

A shake flask being mixed at 200 rpm

All of the above would have equivalent oxygen transfer rate characteristics

The answer is A sparged stirred tank bioreactor being stirred at 200 rpm

35. Molar heat capacity of water in equilibrium with ice at constant pressure is 

Zero

Infinity

40.45 KJ K^-1 mol^-1

75.48 JK^-1 mol^-1

The answer is Zero
36. Ammonium molybdate test is used to detect the presence of 

Borate

Phosphate

Chloride

Bromide


The answer is Phosphate
37. Increasing the temperature of an aqueous solution will cause 

Decrease in Molality

Decrease in Molarity

Decrease in Mole Fraction

Decrease in Percentage by Weight


The answer is Decrease in Molarity

38. IUPAC name of the compound CH3CH(OH)COOH is 

Lactic Acid

A-Hydroxypropanoic Acid

Carboxy Propanol

2-Hydroxypropanoic acid

The answer is 2-Hydroxypropanoic acid
39 Which of the following will not displace hydrogen ? 

Ba

Pb

Ag

Sn


The answer is Ag
40. The amount of heat measure for a reaction in a bomb calorimeter is 

DG

DH

DE

PDV


The answer is DE
41. Which of the following oxides is most amphoteric ? 

Na2O

Al2O3

SO3

P2O5


The answer is Al2O3
42. Which among the following is most basic compound ? 

Benzylamine

Aniline

Acetanilide

P-Nitroaniline

The answer is Benzylamine
43. Which of the following elements form interstitial compounds ? 

Fe

Co

Ni

All of these


The answer is All of these

44 The EAN of Co in K3[Co(NH3)6] is 

34

35

36

37


The answer is 36
45 The position of electron is identified by quantum numbers n and l. (i) n = 4, l = 1 (ii) n = 4, l = 0 (iii) n = 3, l = 2 (iv) n = 3, l = 1 The order of increasing energy from the lowest to highest is 

(iv) < (ii) < (iii) < (i)

(ii) < (iv) < (i) < (iii)

(i) < (iii) < (ii) < (iv)

(iii) < (i) < (iv) < (ii)


The answer is (iv) < (ii) < (iii) < (i)
46. Gutta Percha is 

Trans-polyisoproprene

A synthetic polymer

A very hard material

All are correct


The answer is All are correct
47. Which one of following does not give acetylene with water ? 

CaC2

BaC2

SrC2

Al4C3

The answer is Al4C3
48. Normality of 0.3 M phosphoric acid (H3PO3) is 

0.3

0.6

0.9

0.1

The answer is 0.6
49. ___________ technique is based on the fact that there are variations in the DNA sequence of restriction sites among different individuals and different species. 

Polymer Chain Reaction

Hybridoma

Restriction Fragment Length Polymorphism

None of these


The answer is Restriction Fragment Length Polymorphism
50 Which enzyme forms phosphodiester bonds between adjacent nucleotides and covalently links two individual fragments of double stranded DNA 

Alkaline Phosphates

DNA Polymerase

DNA Liqase

Exonuclease


The answer is DNA Liqase
51 Name the enzyme used to prevent unwanted self-liquation of vector DNA molecules in cleaning procedures 

Alkaline Phosphates

DNA Ligase

DNA Polymerase

Helicases


The answer is Alkaline Phosphates
52. Many of the vector for use in eukaryotic cells are constructed such that they can exist in both the eukaryotic cell and E.coli. Such vectors are known as 

Shuttle Passengers

Shuttle Vectors

Expression Vectors

All of these


The answer is Shuttle Vectors
53 ___________ are constructed by combining certain features of plasmid and the cos sites of the phage lambda 

Hybrides

Cybrids

Cosmids

None of these


The answer is Cosmids
54 ____________ are used as vector to clone DNA fragments of more than 1 Mb in size 

Yeast Artificial Chromosomes

Bacterial Artificial Chromosomes

Plant Chromosomes

All of these


The answer is Yeast Artificial Chromosomes
55 ___________ vector can accommodate upto 300-350 kb of foreign DNA 

YAC

BAC

Plant

Animal
Answer BAC
56 A collection of clones representing the complete genome of an organism is called 

Genomic Library

DNA Library

Inventory

None of these
Answer-Genomic Library
57. Which method is used to introduce recombinant DNA into host cell ? 

Electroporation

Microinjection

Biolistics

All of the above-Answer
58. Who invented Polymerase Chain Reaction ? 

Ely Lilly

Kary Mullis

Sanger

All of these


The answer is Kary Mullis
59 Who developed Dideoxynucleotide Chain termination method ? 

Frederick Sanger and Andrew Coulson

Andrew Coulson and Maxam

Gilbert

All of them

The answer is Frederick Sanger and Andrew Coulson
60 Who coined the term genomics ? 

Winkler

Thomas Roderick

Craig Venter

All of them


The answer is Thomas Roderick
61. ___________ primarily involves high-throughput DNA sequencing followed by assembly, organization and management of DNA sequence 

Structural Genomics

Functional Genomics

Contigs

None of these


The answer is Structural Genomics
62. ____________ deals with the reconstruction of the genome to determine the biological function of genes and gene interaction 

Structural Genomics

Functional Genomics

Random Libraries

Contigs


The answer is Functional Genomics
63 _________ of genomic DNA are constructed in small and edium seat plasmid vector along with genomic shotgun large unseat BAC library 

Random Arrays

Mirco Arrays

Random Libraries

All of these


The answer is Random Libraries
64. BLAST means 

Basic Local Alignment Search Tool

Basic Local Array Search Tool

Basic Linkage Alignment Search Technique

None of them


The answer is Basic Local Alignment Search Tool
65. ___________ is a closed culture system, which contains limited amount of nutrients 

Fed-Batch Culture

Continuous Culture

Batch Culture

All of these

The answer is Batch Culture
66.If a batch culture is continuously or sequentially fed with fresh medium without remaining the growing culture, it is called 

Batch Culture

Fed-Batch Culture

Continuous Culture

Another Culture


The answer is Fed-Batch Culture

67 If the temperature of saturated water is increased infinitesimally at constant entropy, the resulting state of water will be
(A) Liquid 
(B) Liquid – vapor coexistence
(C) Saturated vapor 
(D) Solid

68 Water is flowing under laminar conditions in a pipe of length L . If the diameter of the pipe is 
doubled, for a constant volumetric flow rate, the pressure drop across the pipe
(A) decreases 2 times 
(B) decreases 16 times
(C) increases 2 times 
(D) increases 16 times

70 For uniform laminar flow (in the x -direction) past a flat plate at high Reynolds number, the local boundary layer thickness (? ) varies with the distance along the plate ( x ) as

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